Active Recombinant Human RNASE1 Protein, His-tagged

• Cat.No.: RNASE1-8588H
• Product Overview: Active Recombinant Human RNASE1 Protein(P07998) (Met1-Thr156) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-156 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to hydrolyze yeast RNA. One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm in 15 min when yeast RNA is hydrolyzed at 37°C and pH 7.0. The specific activity is >2.5×10^6 unit/mg.
• Molecular Mass: The recombinant human RNASE1 comprises 139 amino acids and has a predicted molecular mass of 16 kDa. The apparent molecular mass of the protein is approximately 29 ,26 and 22 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RNASE1 ribonuclease, RNase A family, 1 (pancreatic) [ Homo sapiens ]
• Official Symbol: RNASE1
• Synonyms: RNASE1; ribonuclease, RNase A family, 1 (pancreatic); RNS1; ribonuclease pancreatic; RIB-1; RNase 1; RNase A; HP-RNase; RNase upI-1; ribonuclease 1; ribonuclease A; RIB1; MGC12408
• Gene ID: 6035
• mRNA Refseq: NM_002933
• Protein Refseq: NP_002924
• MIM: 180440
• UniProt ID: P07998