"PCNA" Related Products

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Recombinant Human PCNA, His-tagged

Cat. No.: PCNA-175H
Product Overview: Recombinant Human PCNA (Met 1-Ser 261) fused with a polyhistidine tag at the N-terminus, was expressed in Baculovirus-Insect cells.
Description: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Source: Baculovirus-Insect cells
Molecular Mass: The recombinant human PCNA consists of 280 amino acids and has a calculated molecular mass of 31KDa. It migrates as an approximately 36 KDa band in SDS-PAGE under reducing conditions.
Predicted N terminal: Met
Purity: > 90 % as determined by SDS-PAGE
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Formulation: Lyophilized from sterile 50mM Na3PO4, 300mM NaCl, 10%glycerol, pH 7.0, 2mM DTT
Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.
OfficialSymbol: PCNA
Gene Name: PCNA proliferating cell nuclear antigen [ Homo sapiens ]
Synonyms: MGC8367; PCNA; Proliferating cell nuclear antigen; Cyclin; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
Gene ID: 5111
mRNA Refseq: NM_002592
Protein Refseq: NP_002583
MIM: 176740
UniProt ID: P12004
Chromosome Location: 20pter-p12
Pathway: Base excision repair; Cell cycle; DNA replication; Mismatch repair; Nucleotide excision repair; Cell Cycle, Mitotic; DNA Repair; DNA Replication; Telomere Maintenance
Function: DNA binding; DNA polymerase processivity factor activity; MutLalpha complex binding; dinucleotide insertion or deletion binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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