Active Recombinant Mouse Nt5e, His-tagged

• Cat.No.: Nt5e-250M
• Product Overview: Recombinant Mouse Nt5e (Trp29-Lys549) fused with 6­His tag at C­terminal, was expressed in CHO Cells.

Specification

• Description: 5''-nucleotidase(5''-NT), also known as ecto-5''-nucleotidaseor CD73 (Cluster ofDifferentiation 73), is an enzymethat in humans is encoded by theNT5E gene. CD73 commonly serves to convert AMP toadenosine.
• Source: CHO Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Trp29
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Bio-activity: Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is >10,000 pmol/min/µg.
• Molecular Mass: 59 kDa
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: Nt5e 5'' nucleotidase, ecto [ Mus musculus (house mouse) ]
• Official Symbol: Nt5e
• Synonyms: Nt5e; NT; Nt5; eNT; CD73; AI447961; 2210401F01Rik; 5'' nucleotidase, ecto; 5''-nucleotidase; 5''-NT; ecto-5''-nucleotidase; EC 3.1.3.5
• Gene ID: 23959
• mRNA Refseq: NM_011851
• Protein Refseq: NP_035981
• UniProt ID: Q61503
• Chromosome Location: 9; 9 E3.2
• Pathway: Metabolism of nucleotides; Nicotinate and nicotinamide metabolism; Purine metabolism
• Function: 5''-nucleotidase activity; ferrous iron binding; hydrolase activity, acting on ester bonds

Reviews

11/20/2022

the manufacturer's support plays a crucial role in the successful implementation of trials involving the NT5E protein.

09/01/2017

The manufacturer maintains stringent quality control measures to ensure the purity and stability of the protein, minimizing experimental variability and maximizing the accuracy of the data obtained.

01/25/2017

Their responsiveness to inquiries and willingness to address concerns contribute to a smooth and productive research experience.

Q&As

Can NT5E be used as a biomarker for certain diseases?

Yes, elevated levels of NT5E have been observed in various diseases, making it a potential biomarker for conditions such as cancer and autoimmune disorders.

How does NT5E contribute to inflammation?

NT5E-generated adenosine has anti-inflammatory effects, and modulating NT5E activity is being explored as a potential therapeutic strategy for inflammatory conditions.

Is NT5E associated with autoimmune diseases?

Yes, NT5E is implicated in autoimmune diseases, and targeting its activity is being investigated for potential therapeutic interventions.

Are there any FDA-approved drugs targeting NT5E for cancer treatment?

As of my last knowledge update in January 2022, there were no FDA-approved drugs specifically targeting NT5E, but research in this area is ongoing.

How is NT5E linked to neurological disorders?

NT5E-generated adenosine is neuroprotective, and research is exploring its potential in the treatment of neurodegenerative disorders.