Native Bovine CAPN2

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Native Bovine CAPN2

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Cat.No. : CAPN2-350B
Product Overview : CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.
Source : Bovine Myocardium.
Species : Bovine
Form : 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4.
Molecular Mass : CAPN2 consists of an 80 kDa large subunit and a 30 kDa small subunit.
Purity : Greater than 90% as determined by SDS-PAGE.
Applications : his protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: A- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1,000). B- Add 1μg of protein to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.
Stability : CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name : CAPN2 calpain 2, (m/II) large subunit [ Bos taurus (cattle) ]
Official Symbol : CAPN2
Synonyms : calpain-2 catalytic subunit; CANP 2; M-calpaincalpain M-type; millimolar-calpain; calpain-2 large subunit; calpain, large polypeptide L2; calcium-activated neutral proteinase 2
Gene ID : 281662
mRNA Refseq : NM_001103086
Protein Refseq : NP_001096556
UniProt ID : Q27971
Chromosome Location : chromosome: 16
Pathway : Alzheimer"s disease, organism-specific biosystem; Alzheimer"s disease, conserved biosystem; Apoptosis, organism-specific biosystem
Function : calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; calcium-dependent cysteine-type endopeptidase activity

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