Recombinant human MDM2, His-tagged

• Cat.No.: MDM2-350H
• Product Overview: Recombinant Human MDM2(1-118) was expressed in E. coli cells using an N-terminal His tag.

Specification

• Description: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1 mM PMST, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~17.5 kDa
• AA Sequence: 1-118
• Purity: > 85%
• Applications: Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Publication:
  Design, synthesis and in vitro and in vivo antitumour activity of 3-benzylideneindolin-2-one derivatives, a novel class of small-molecule inhibitors of the MDM2–p53 interaction. (2014)

Gene Information

• Gene Name: MDM2 MDM2 oncogene, E3 ubiquitin protein ligase [ Homo sapiens (human) ]
• Official Symbol: MDM2
• Synonyms: MDM2; HDMX; hdm2; ACTFS; MDM2 oncogene, E3 ubiquitin protein ligase; E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; Mdm2, p53 E3 ubiquitin protein ligase homolog; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; EC 6.3.2.-
• Gene ID: 4193
• mRNA Refseq: NM_002392
• Protein Refseq: NP_002383
• MIM: 164785
• UniProt ID: Q00987
• Chromosome Location: 12q14.3-q15
• Pathway: AKT phosphorylates targets in the cytosol; Androgen receptor signaling pathway; Cell Cycle
• Function: enzyme binding; p53 binding; peroxisome proliferator activated receptor binding