Active Recombinant Human MMP9
Cat.No. : | MMP9-166H |
Product Overview : | Active recombinant matrix metalloproteinase-9 (MMP-9, gelatinase B, 92 kDa type IV collagenase) cloned from human cDNA. The enzyme consists of residues Phe107-Pro449 (NM_004994), which comprises the catalytic/fibronectin domain of human MMP-9, with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-9 which lacks the C-terminal hemopexin domain. Activity toward its targets, such as gelatin, casein, or peptide substrates, is unaffected. |
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- Gene Information
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Description : | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP''s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. |
Source : | E. coli |
Species : | Human |
Form : | Liquid. In 50mM TRIS, pH 7.5, containing 5mM calcium chloride, 300mM sodium chloride, 20µM zinc chloride, 0.5% Brij-35 and 30% glycerol. |
Bio-activity : | XX U/µg. One U=100pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt as substrate. Preincubation of MMP-9 catalytic domain at 1.3nM with the broad-spectrum inhibitor GM6001 at 30nM for 1 hour inhibits enzymatic activity by 97%. |
Molecular Mass : | 39 kDa |
Purity : | ≥95% (SDS-PAGE) |
Applications : | Study enzyme kinetics, cleave target substrates, and screen for inhibitors. |
Storage : | -80°C |
Concentration : | 0.5µg/µl |
Gene Name : | MMP9 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) [ Homo sapiens (human) ] |
Official Symbol : | MMP9 |
Synonyms : | MMP9; GELB; CLG4B; MMP-9; MANDP2; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); EC 3.4.24.35 |
Gene ID : | 4318 |
mRNA Refseq : | NM_004994 |
Protein Refseq : | NP_004985 |
MIM : | 120361 |
UniProt ID : | P14780 |
Chromosome Location : | 20q11.2-q13.1 |
Pathway : | AGE/RAGE pathway; Angiogenesis; Bladder cancer |
Function : | collagen binding; metalloendopeptidase activity; protein binding |
Products Types
◆ Recombinant Protein | ||
MMP9-560H | Recombinant Human MMP9 Protein, MYC/DDK-tagged | +Inquiry |
Mmp9-37H | Active Recombinant Rat Mmp9 protein (20-708aa), C-6×His-tagged | +Inquiry |
MMP9-1131P | Recombinant Pig MMP9 Protein, His-tagged | +Inquiry |
MMP9-03H | Active Recombinant Pre-activated Human MMP9 Protein | +Inquiry |
MMP9-21B | Recombinant Bovine MMP9 protein(107-712aa), His-tagged | +Inquiry |
◆ Native Protein | ||
MMP9-30035TH | Native Human MMP9 | +Inquiry |
MMP9-39H | Native Human MMP-9/Lipocalin Complex | +Inquiry |
MMP9-29698TH | Native Human MMP9 | +Inquiry |
◆ Lysates | ||
MMP9-2026MCL | Recombinant Mouse MMP9 cell lysate | +Inquiry |
MMP9-1940RCL | Recombinant Rat MMP9 cell lysate | +Inquiry |
MMP9-2560HCL | Recombinant Human MMP9 cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-3100 | MMP-9 Fluorimetric Assay Kit | +Inquiry |
Kit-2130 | MMP-9 Inhibitor Screening Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionDysregulated MMP9 expression is associated with cancer invasion and metastasis, as it enables tumor cells to degrade the extracellular matrix and invade surrounding tissues.
Polymorphisms in the MMP9 gene can influence enzyme activity, potentially affecting the individual's susceptibility to certain diseases and the progression of pathological conditions.
MMP9 facilitates tissue remodeling by cleaving structural proteins in the extracellular matrix, such as collagen and gelatin, leading to changes in tissue architecture.
MMP9 contributes to the breakdown of the blood-brain barrier by degrading tight junction proteins, potentially exacerbating neurological disorders and neuroinflammation.
MMP9 plays a role in angiogenesis by promoting the release and activation of growth factors sequestered in the extracellular matrix, facilitating the formation of new blood vessels.
Customer Reviews (3)
Write a reviewThe superior quality of this recombined protein has significantly enhanced the precision of our research outcomes.
Fast shipping! Ordered this product, and it arrived well-packaged and ready for use in our experiments.
Customer service went above and beyond to accommodate our specific needs, ensuring a smooth purchasing experience.
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