Recombinant Rat Mmp9 protein, His-tagged

• Cat.No.: Mmp9-436R

Specification

• Product overview: Recombinant Rat Mmp9 (P50282) aa. (Gly213~Met422) fused with His tag was produced in Yeast cells.
• Description: metalloproteinase involved in extracellular matrix remodeling and bone resorption
• Source: Yeast
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 24.3kDa; Accurate Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly213~Met422
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 4.9.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Mmp9 matrix metallopeptidase 9 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Mmp9
• Synonyms: Mmp9; matrix metallopeptidase 9; 92 kDa gelatinase; 92-kDa type IV collagenase; GELB; MMP-9; gelatinase B; matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)
• Gene ID: 81687
• mRNA Refseq: NM_031055.1
• Protein Refseq: NP_112317.1
• UniProt ID: P50282

Reviews

05/03/2020

The superior quality of this recombined protein has significantly enhanced the precision of our research outcomes.

08/15/2019

Fast shipping! Ordered this product, and it arrived well-packaged and ready for use in our experiments.

01/21/2018

Customer service went above and beyond to accommodate our specific needs, ensuring a smooth purchasing experience.

Q&As

What are the consequences of dysregulated MMP9 expression in cancer progression?

Dysregulated MMP9 expression is associated with cancer invasion and metastasis, as it enables tumor cells to degrade the extracellular matrix and invade surrounding tissues.

What is the impact of MMP9 polymorphisms on enzyme activity?

Polymorphisms in the MMP9 gene can influence enzyme activity, potentially affecting the individual's susceptibility to certain diseases and the progression of pathological conditions.

Can you explain the role of MMP9 in tissue remodeling at the molecular level?

MMP9 facilitates tissue remodeling by cleaving structural proteins in the extracellular matrix, such as collagen and gelatin, leading to changes in tissue architecture.

What is the role of MMP9 in the breakdown of the blood-brain barrier during neurological disorders?

MMP9 contributes to the breakdown of the blood-brain barrier by degrading tight junction proteins, potentially exacerbating neurological disorders and neuroinflammation.

How does MMP9 contribute to angiogenesis at the molecular level?

MMP9 plays a role in angiogenesis by promoting the release and activation of growth factors sequestered in the extracellular matrix, facilitating the formation of new blood vessels.