Recombinant Rhesus S100B protein

• Cat.No.: S100B-544R
• Product Overview: Recombinant Rhesus S100B protein was expressed in Escherichia coli.

Specification

• Description: S100B belongs to the S100 family, which containing 2 EF-hand calcium-binding motifs. In humans, S100B protein is encoded by the S100P gene located at 4q16, but genes that encode other numbers of s100 family proteins are almost located at 1q21 as a cluster. S100B is glial-specific and is expressed primarily by astrocytes. Not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein.
• Source: E.coli
• Species: Rhesus
• Form: Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
• Molecular Mass: Approximately 10.6 kDa, a single non-glycosylated polypeptide chain containing 91 amino acids
• Protein length: 91
• AA Sequence: SELEKAMVALIDVFHQYSGREGDKH KLKKSELKELINNELSHFLEEIKEQ EVVDKVMETLDSDGDGECDFQEFMA FVAMVTTACHEFFEHE
• Endotoxin: Less than 1 EU/μg of rRhS100B as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: S100B
• Official Symbol: S100B
• Synonyms: Protein S100-P, Migration-inducing gene 9 protein, MIG9, Protein S100-E, S100 calcium-binding protein P
• Gene ID: 708117
• mRNA Refseq: NM_001260526.1
• Protein Refseq: NP_001247455.1
• UniProt ID: F7ANQ9

Reviews

03/15/2022

One major advantage of S100B protein is its ability to contribute to the understanding of diseases related to aberrant cell cycle regulation, including cancer.

09/29/2019

The S100B protein demonstrates exceptional quality, making it an ideal candidate to fulfill my experimental requirements.

10/23/2018

The manufacturer of S100B protein plays a crucial role in supporting my research efforts.

Q&As

How does S100B help in the diagnosis of traumatic brain injury (TBI)?

Elevated S100B levels in the blood can indicate TBI, and its measurement can aid in TBI diagnosis and severity assessment.

What conditions can S100B be used to diagnose?

S100B can be used to diagnose brain injuries, such as traumatic brain injury and stroke.

How is S100B measured in clinical settings?

S100B levels are typically measured through a blood test.

What precautions should be taken when interpreting S100B results?

Results should be interpreted in the context of the patient's clinical history and other diagnostic tests.

How is S100B used in the context of neurodegenerative diseases?

S100B may help in monitoring disease progression and severity in conditions like Alzheimer's disease.