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Recombinant Rat Fgf21

Cat.No.: Fgf21-589R
Product Overview: Recombinant Rat Fgf21 produced in E. coli is approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
Description: Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and murine FGF-21, and it show activity on murine and human cells.
Source: E. coli
Species: Rat
Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.
Bio-activity: Fully biologically active when compared to standard. The ED 50 determined by a cell proliferation assay using murine NIH/3T3 cells is less than 700 ng/ml, corresponding to a specific activity of >1.4 × 10^3 IU/mg in the presence of 5 μg/ml of rMuKlotho-β.
Molecular Mass: 19.7 kDa
AA Sequence: AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS
Endotoxin: Less than 1 EU/μg of rRtFGF-21 as determined by LAL method.
Purity: >95% by SDS-PAGE and HPLC analyses.
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name: Fgf21 fibroblast growth factor 21 [ Rattus norvegicus ]
Official Symbol: Fgf21
Synonyms: FGF21; fibroblast growth factor 21;
Gene ID: 170580
mRNA Refseq: NM_130752
Protein Refseq: NP_570108
Pathway: MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; Melanoma, organism-specific biosystem; Melanoma, conserved biosystem; Pathways in cancer, organism-specific biosystem; Regulation of Actin Cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, organism-specific biosystem;
Function: fibroblast growth factor receptor binding; growth factor activity;

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