Recombinant Human PI4KA, GST-tagged

• Cat.No.: PI4KA-117H
• Product Overview: Recombinant full-length human PI4KA was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PI4KA encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by PI4KA gene is a type III enzyme that is not inhibited by adenosine. PI4KA is highly expressed in placenta and brain. PI4KA is associated with the membranes of Golgi vesicles and vacuoles.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~120 kDa
• Purity: >70% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: PI4KA phosphatidylinositol 4-kinase, catalytic, alpha [ Homo sapiens ]
• Official Symbol: PI4KA
• Synonyms: PI4KA; phosphatidylinositol 4-kinase, catalytic, alpha; PIK4CA; phosphatidylinositol 4-kinase alpha; PI4K ALPHA; pi4K230; PI4-kinase alpha; ptdIns-4-kinase alpha; phosphatidylinositol 4-kinase 230; phosphatidylinositol 4-kinase, type III, alpha; PI4K-ALPHA; FLJ16556
• Gene ID: 5297
• mRNA Refseq: NM_002650
• Protein Refseq: NP_002641
• MIM: 600286
• UniProt ID: P42356
• Chromosome Location: 22q11.21
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Inositol phosphate metabolism, PI=>
• Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; nucleotide binding; phosphatidylinositol 3-kinase activity; protein binding