||Recombinant Human PCSK9 MS Standard Protein, C13 and N15-labeled (PCSK9, Heavy Labeled) Gln 31 - Gln 692 (Accession # AAI66619) was produced in human 293 cells (HEK293).
||Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).[2-3]
||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
||PCSK9, Heavy Labeled, fused with 6×His tag at the C-terminus, has a calculated MW of 75.1 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 20 kDa and 62 kDa due to glycosylation and proteolytic digestion.
||Less than 1.0 EU per μg of the PCSK9, Heavy Labeled by the LAL method.
||>97% as determined by SDS-PAGE.
||Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).