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Recombinant Human ADAM15 cell lysate

Cat.No. : ADAM15-001HCL
Product Overview : Human ADAM15 derived in CHO cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : CHO cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : ADAM15 ADAM metallopeptidase domain 15 [ Homo sapiens ]
Official Symbol : ADAM15
Synonyms : ADAM15; ADAM metallopeptidase domain 15; a disintegrin and metalloproteinase domain 15 (metargidin); disintegrin and metalloproteinase domain-containing protein 15; MDC15; metargidin; MDC-15; ADAM 15; metalloprotease RGD disintegrin protein; metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15;
Gene ID : 8751
mRNA Refseq : NM_003815
Protein Refseq : NP_003806
MIM : 605548
UniProt ID : Q13444
Chromosome Location : 1q21.3
Function : SH3 domain binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; protein binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (4)

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In addition to breast cancer research, what other diseases is ADAM15 currently used to study? 04/05/2022

ADAM15 used in studies of liver and bladder cancers.

I want to buy ADAM15-related proteins, do you provide other proteins? 10/09/2021

We offer MMP9 and MMP2 proteins in addition to ADAM15 proteins.

Does ADAM15 affect H460 expression? 06/26/2020

ADAM15 affects the proliferation of H460 cells by regulating PI3K-AKT and MAPK-ERK signaling pathways.

Does adding GST tag have any effect on protein? 12/13/2019

Addition of GST tags can accelerate the solubilization of certain insoluble proteins.

Customer Reviews (2)

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Reviews
09/04/2021

    The purchased protein is suitable for WB experiments and I am satisfied with the results.

    05/17/2020

      The technical expert communicated with me in time to understand the progress of my experiment and gave me suggestions for improvement.

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