Recombinant Mouse FLT3 cell lysate

• Cat.No.: FLT3-970MCL
• Product Overview: Mouse FLT3 / FLK2 / CD135 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: Flt3 FMS-like tyrosine kinase 3 [ Mus musculus ]
• Official Symbol: FLT3
• Synonyms: FLT3; FMS-like tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; fetal liver kinase 2; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04; MGC124377; MGC124378
• Gene ID: 14255
• mRNA Refseq: NM_010229
• Protein Refseq: NP_034359
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: ATP binding; glucocorticoid receptor binding; kinase activity; nucleotide binding; phosphatidylinositol 3-kinase binding; protein binding; protein complex binding; protein kinase activity; protein tyrosine kinase activity; receptor activity; transferase activity; transferase activity, transferring phosphorus-containing groups; transmembrane receptor protein tyrosine kinase activity

Reviews

08/01/2021

Excellent product, great price.

12/22/2020

Satisfied, will order again.

09/14/2020

Quick response, helpful service.

Q&As

How is FLT3 implicated in acute myeloid leukemia (AML)?

FLT3 is frequently mutated in AML, contributing to uncontrolled cell growth and poor prognosis.

In which cells or tissues is FLT3 predominantly expressed?

FLT3 is predominantly expressed in hematopoietic stem cells and progenitor cells.

How does FLT3 contribute to hematopoiesis?

It is essential for hematopoiesis, particularly in the proliferation of early progenitor cells.

Are there therapeutic strategies targeting FLT3 for disease treatment?

Therapeutic strategies include FLT3 inhibitors, showing effectiveness in treating AML patients with FLT3 mutations.

What is the primary biological function of FLT3 in cells?

FLT3 is a receptor tyrosine kinase that plays a crucial role in cell survival, proliferation, and differentiation.

What role does FLT3 play in the proliferation and differentiation of hematopoietic stem cells?

It stimulates the proliferation and differentiation of hematopoietic stem cells, guiding their development into various blood cell types.

What are the common mutations found in FLT3 associated with diseases?

Common mutations include internal tandem duplications (ITD) and tyrosine kinase domain (TKD) mutations, leading to constitutive activation of FLT3.