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Recombinant Human MGAT5 cell lysate

Cat.No. : MGAT5-2067HCL
Product Overview : Human MGAT5 / GGNT5 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : MGAT5 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase [ Homo sapiens ]
Official Symbol : MGAT5
Synonyms : MGAT5; mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; GNT V; glcNAc-T V; N-acetylglucosaminyl-transferase V; mannoside acetylglucosaminyltransferase 5; alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; GNT-V; GNT-VA; FLJ31899; FLJ43217; DKFZp686B24166;
Gene ID : 4249
mRNA Refseq : NM_002410
Protein Refseq : NP_002401
MIM : 601774
UniProt ID : Q09328
Chromosome Location : 2q21
Pathway : Asparagine N-linked glycosylation, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan antennae elongation, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; N-glycan antennae elongation in the medial/trans-Golgi, organism-specific biosystem;
Function : alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What role does MGAT5 play in immune responses? 07/24/2022

MGAT5 modulates immune responses.

What is the primary role of MGAT5 in glycoprotein synthesis? 12/24/2021

MGAT5 is key for glycoprotein glycosylation.

How does MGAT5 contribute to cell signaling and communication? 09/09/2021

It affects cell signaling by altering glycosylation patterns.

What is the impact of altered MGAT5 activity on cancer progression? 09/06/2021

Altered MGAT5 activity is linked to cancer progression.

How do genetic variations in MGAT5 affect cellular functions? 03/22/2020

Genetic changes in MGAT5 impact glycosylation and cell processes.

What potential therapeutic applications arise from targeting MGAT5 in diseases? 01/29/2020

Targeting MGAT5 could treat diseases involving abnormal glycosylation.

How does MGAT5 interact with other enzymes in glycosylation pathways? 06/08/2019

MGAT5 collaborates with other enzymes in glycosylation.

Customer Reviews (3)

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02/19/2023

    Trusted for research integrity, a dependable research collaborator.

    10/07/2020

      Efficient service, accelerates research progress.

      11/24/2018

        Outstanding accuracy, an essential part of our research.

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