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Recombinant Human TTR cell lysate

Cat.No. : TTR-2149HCL
Product Overview : Human Transthyretin / TTR / Prealbumin / PALB derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TTR transthyretin [ Homo sapiens ]
Official Symbol : TTR
Synonyms : TTR; transthyretin; PALB, prealbumin, amyloidosis type I; HsT2651; ATTR; carpal tunnel syndrome 1; thyroxine-binding prealbumin; prealbumin, amyloidosis type I; CTS; CTS1; PALB; TBPA;
Gene ID : 7276
mRNA Refseq : NM_000371
Protein Refseq : NP_000362
MIM : 176300
UniProt ID : P02766
Chromosome Location : 18q12.1
Pathway : Amyloids, organism-specific biosystem; Disease, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem;
Function : hormone activity; hormone binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are the main physiologic functions of TTR? 07/14/2022

TTR is involved in the transport of thyroxine and retinol and plays a role in processes such as protein degradation.

How are RAGE proteins related to TTR proteins? 11/23/2021

RAGE proteins interact with TTR proteins and are involved in the regulation of TRR expression.

In which animals is TTR expressed? 06/07/2021

TTR proteins are highly conserved and are found in several vertebrates, such as humans, rats, sheep, and chickens.

Where is TTR primarily synthesized? 03/05/2021

TTR is mainly synthesized in the liver.

TTR is closely linked to research on what diseases 10/22/2020

TTR is associated with a variety of diseases including amyloidosis TTR (ATTR), dementia (AD) and neurological damage.

Customer Reviews (3)

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Reviews
07/14/2022

    Timely delivery, so I got the desired experimental results in the shortest possible time.

    11/26/2021

      I have a dedicated staff member who contacts me and keeps me informed on the progress of custom protein production.

      01/02/2021

        The purchased product information is in line with the advertising campaign and is conducive to obtaining accurate experimental results.

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