Recombinant Human CLU cell lysate
Cat.No. : | CLU-2198HCL |
Product Overview : | Human CLU / Clusterin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | CLU clusterin [ Homo sapiens ] |
Official Symbol : | CLU |
Synonyms : | CLU; clusterin; APOJ, CLI, clusterin (complement lysis inhibitor, SP 40,40, sulfated glycoprotein 2, testosterone repressed prostate message 2, apolipoprotein J); apolipoprotein J; complement lysis inhibitor; KUB1; SGP 2; SP 40; sulfated glycoprotein 2; testosterone repressed prostate message 2; TRPM 2; ku70-binding protein 1; aging-associated protein 4; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2; CLI; AAG4; APOJ; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; MGC24903; |
Gene ID : | 1191 |
mRNA Refseq : | NM_001831 |
Protein Refseq : | NP_001822 |
MIM : | 185430 |
UniProt ID : | P10909 |
Chromosome Location : | 8p21-p12 |
Pathway : | Hemostasis, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Platelet degranulation, organism-specific biosystem; Response to elevated platelet cytosolic Ca2+, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem; |
Function : | NOT ATPase activity; misfolded protein binding; misfolded protein binding; protein binding; ubiquitin protein ligase binding; |
Products Types
◆ Recombinant Protein | ||
CLU-746R | Recombinant Rhesus Macaque CLU Protein, His (Fc)-Avi-tagged | +Inquiry |
CLU-1130R | Recombinant Rat CLU Protein, His (Fc)-Avi-tagged | +Inquiry |
CLU-621H | Recombinant Human CLU Protein, His (Fc)-Avi-tagged | +Inquiry |
CLU-803H | Recombinant Human CLU Protein, His&GST-tagged | +Inquiry |
CLU-3277H | Recombinant Human CLU Protein, MYC/DDK-tagged | +Inquiry |
◆ Native Protein | ||
CLU-19H | Native Human Clusterin Protein | +Inquiry |
CLU-67H | Native Human Clusterin | +Inquiry |
◆ Lysates | ||
CLU-2195MCL | Recombinant Mouse CLU cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionCLU has anti-inflammatory properties and modulates immune responses. Its involvement in autoimmunity is linked to its ability to regulate immune cell functions and cytokine production.
CLU plays a role in regulating cell survival and apoptosis by interacting with key cellular components, including Bcl-2 family members and caspases, modulating apoptotic pathways.
CLU modulates cell adhesion and migration, contributing to tissue remodeling and repair. Its interactions with extracellular matrix components influence cellular behaviors in wound healing and tissue homeostasis.
CLU is implicated in neurodegenerative disorders, particularly Alzheimer's disease. Its involvement in processes such as amyloid-beta aggregation and clearance influences disease progression.
CLU interacts with lipids, participating in lipid transport and metabolism. Its role in lipoprotein remodeling and cholesterol homeostasis links it to cardiovascular health.
Customer Reviews (3)
Write a reviewImpressed with the quality control. This product is a gem in our lab experiments.
Smooth transactions and swift delivery. Our lab supplies are incomplete without this protein.
Our lab manager introduced us to this product, and we're never going back. Essential for our studies.
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