Recombinant Human PRMT5 cell lysate
Cat.No. : | PRMT5-2577HCL |
Product Overview : | Human PRMT5 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | PRMT5 protein arginine methyltransferase 5 [ Homo sapiens ] |
Official Symbol : | PRMT5 |
Synonyms : | PRMT5; protein arginine methyltransferase 5; HRMT1L5, SKB1, skb1 (S. pombe) homolog , SKB1 homolog (S. pombe); protein arginine N-methyltransferase 5; SKB1Hs; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5; JBP1; SKB1; IBP72; HRMT1L5; |
Gene ID : | 10419 |
mRNA Refseq : | NM_001039619 |
Protein Refseq : | NP_001034708 |
MIM : | 604045 |
UniProt ID : | O14744 |
Chromosome Location : | 14q11.2 |
Pathway : | E2F transcription factor network, organism-specific biosystem; Gene Expression, organism-specific biosystem; Metabolism of non-coding RNA, organism-specific biosystem; RNA transport, organism-specific biosystem; RNA transport, conserved biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem; p53 pathway, organism-specific biosystem; |
Function : | histone-arginine N-methyltransferase activity; methyltransferase activity; protein binding; protein-arginine omega-N symmetric methyltransferase activity; ribonucleoprotein complex binding; transferase activity; |
Products Types
◆ Recombinant Protein | ||
PRMT5-1771H | Recombinant Human PRMT5 Protein, His (Fc)-Avi-tagged | +Inquiry |
PRMT5-7125M | Recombinant Mouse PRMT5 Protein, His (Fc)-Avi-tagged | +Inquiry |
PRMT5-3432R | Recombinant Rhesus Macaque PRMT5 Protein, His (Fc)-Avi-tagged | +Inquiry |
Prmt5-5133M | Recombinant Mouse Prmt5 Protein, Myc/DDK-tagged | +Inquiry |
PRMT5-3004H | Recombinant Human PRMT5 Protein, MYC/DDK-tagged | +Inquiry |
◆ Assay kits | ||
Kit-1858 | PRMT5 Chemiluminescent Assay Kit | +Inquiry |
Kit-1859 | PRMT5 Homogeneous Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (5)
Ask a questionYes, there are ongoing clinical trials investigating the efficacy of PRMT5 inhibitors in various cancer types.
PRMT5 influences cellular differentiation by regulating the expression of genes involved in the process.
Yes, there is interest in exploring combination therapies involving PRMT5 inhibitors with other anticancer agents to enhance treatment efficacy.
PRMT5 methylates histone proteins, leading to changes in chromatin structure and gene expression, thus playing a crucial role in epigenetic regulation.
PRMT5 has been implicated in neurodegenerative diseases, and understanding its role may provide insights into developing therapies for conditions like Alzheimer's and Parkinson's disease.
Customer Reviews (3)
Write a reviewGreat performance in ELISA.
Its purity and functionality ensure precise and reproducible results, enabling me to achieve my research objectives with confidence.
The PRMT5 protein demonstrates exceptional quality, making it an ideal candidate to fulfill my experimental requirements.
Ask a Question for All PRMT5 Products
Required fields are marked with *
My Review for All PRMT5 Products
Required fields are marked with *
Inquiry Basket