"TOMM20" Related Products

Recombinant Human TOMM20 293 Cell Lysate

Cat.No.: TOMM20-872HCL
Description: Antigen standard for translocase of outer mitochondrial membrane 20 homolog (yeast) (TOMM20), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: TOMM20 translocase of outer mitochondrial membrane 20 homolog (yeast) [ Homo sapiens ]
Official Symbol: TOMM20
Synonyms: TOMM20; translocase of outer mitochondrial membrane 20 homolog (yeast); mitochondrial import receptor subunit TOM20 homolog; KIAA0016; MAS20; MOM19; TOM20; translocase of outer mitochondrial membrane 20 homolog type II; mitochondrial 20 kDa outer membrane protein; outer mitochondrial membrane receptor Tom20; MGC117367;
Gene ID: 9804
mRNA Refseq: NM_014765
Protein Refseq: NP_055580
MIM: 601848
UniProt ID: Q15388
Chromosome Location: 1q42
Pathway: Metabolism of proteins, organism-specific biosystem; Mitochondrial Protein Import, organism-specific biosystem;

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