Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human NME1 293 Cell Lysate

Cat.No. : NME1-3792HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : NME1 non-metastatic cells 1, protein (NM23A) expressed in [ Homo sapiens ]
Official Symbol : NME1
Synonyms : NME1; non-metastatic cells 1, protein (NM23A) expressed in; nucleoside diphosphate kinase A; NM23; NM23 H1; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; NB; AWD; NBS; GAAD; NDKA; NDPKA; NDPK-A; NM23-H1;
Gene ID : 4830
mRNA Refseq : NM_000269
Protein Refseq : NP_000260
MIM : 156490
UniProt ID : P15531
Chromosome Location : 17q21.33
Pathway : Arf6 downstream pathway, organism-specific biosystem; Arf6 trafficking events, organism-specific biosystem; E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Purine metabolism, organism-specific biosystem;
Function : ATP binding; GTP binding; deoxyribonuclease activity; gamma-tubulin binding; identical protein binding; kinase activity; magnesium ion binding; nucleoside diphosphate kinase activity; nucleotide binding; protein binding; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
What role does NME1 play in DNA repair and the maintenance of genomic stability? 12/05/2022

NME1 plays a role in DNA repair, contributing to genomic stability.

How does NME1 influence signal transduction in response to cellular stress? 01/30/2022

NME1 modulates cellular responses to stress, affecting signal transduction pathways.

What is the impact of NME1 on apoptosis and cell survival pathways? 05/09/2020

NME1 influences apoptosis and cell survival, impacting cell fate decisions.

How do alterations in NME1 expression or activity affect drug resistance in cancer therapy? 11/05/2019

Changes in NME1 can impact drug resistance in cancer, affecting treatment efficacy.

What are the mechanisms by which NME1 modulates the immune response, especially in tumor immunity? 10/21/2018

NME1 regulates the immune response, particularly in tumor immunity.

How does NME1 contribute to the metastatic potential of cancer cells? 07/03/2018

NME1 affects the metastatic behavior of cancer cells, influencing their spread.

How does NME1 regulate nucleotide metabolism and cellular energy balance? 04/20/2018

NME1 is involved in nucleotide metabolism, crucial for maintaining cellular energy balance.

Customer Reviews (3)

Write a review
Reviews
04/02/2022

    Critical expertise in protein engineering, unmatched in quality.

    10/28/2021

      Reliable protein crystallization services, consistently yields exceptional results.

      12/23/2018

        Efficient protein purification, consistent yields, cost-effective for research.

        Ask a Question for All NME1 Products

        Required fields are marked with *

        My Review for All NME1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends