"MAP4K4" Related Products

Recombinant Human MAP4K4 293 Cell Lysate

Cat.No.: MAP4K4-4500HCL
Description: Antigen standard for mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MAP4K4 mitogen-activated protein kinase kinase kinase kinase 4 [ Homo sapiens ]
Official Symbol: MAP4K4
Synonyms: MAP4K4; mitogen-activated protein kinase kinase kinase kinase 4; FLH21957; hepatocyte progenitor kinase like/germinal center kinase like kinase; HGK; HPK/GCK like kinase; NIK; MEK kinase kinase 4; nck-interacting kinase; HPK/GCK-like kinase HGK; Ste20 group protein kinase HGK; MAPK/ERK kinase kinase kinase 4; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; MEKKK4; FLJ10410; FLJ20373; FLJ90111; KIAA0687;
Gene ID: 9448
mRNA Refseq: NM_001242559
Protein Refseq: NP_001229488
MIM: 604666
UniProt ID: O95819
Chromosome Location: 2q11.2-q12
Pathway: Ceramide signaling pathway, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; EPHB forward signaling, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem;
Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; small GTPase regulator activity;

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