Recombinant Human MAP2 293 Cell Lysate

• Cat.No.: MAP2-4512HCL

Specification

• Description: Antigen standard for microtubule-associated protein 2 (MAP2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: MAP2 microtubule-associated protein 2 [ Homo sapiens ]
• Official Symbol: MAP2
• Synonyms: MAP2; microtubule-associated protein 2; MAP2A; MAP2B; MAP2C; MAP-2; DKFZp686I2148
• Gene ID: 4133
• mRNA Refseq: NM_001039538
• Protein Refseq: NP_001034627
• MIM: 157130
• UniProt ID: P11137
• Chromosome Location: 2q34-q35
• Pathway: LKB1 signaling events, organism-specific biosystem; MAPK Cascade, organism-specific biosystem
• Function: beta-dystroglycan binding; calmodulin binding; protein binding; structural molecule activity

Reviews

04/11/2023

I've been using them for several years now and they've been stable and I trust this product a lot.

02/02/2022

It is very suitable for biological activity experiments, and the activity is good.

05/19/2020

It can meet the needs of daily experiments and has good expression effect.

Q&As

How about the possible aspects of future research on MAP2?

Future research on MAP2 may involve its mechanism of action in neurological diseases, its interaction with other proteins, etc.

What is the relationship between MAP2 and neurological disorders?

MAP2 may be associated with certain neurological diseases, such as Alzheimer's disease and Parkinson's disease. However, there is still insufficient research on this aspect.

What are some of the current controversies or unresolved issues in the study of MAP2?

There are still many controversial and unresolved issues in the current research on MAP2, such as its mechanism of action in neurological diseases.

How can the function of MAP2 be studied experimentally?

The effects of MAP2 on neuronal structure and function, as well as its role in neurological diseases, can be studied through experimental methods such as cell culture and animal models.

How about the distribution of MAP2 in neurons?

MAP2 is unevenly distributed in neurons and is mainly concentrated in axons and dendrites, with a higher density in dendrites.

How about the relationship between MAP2 and learning Xi and memory?

The MAP2 may affect the process of learning Xi and memory by affecting the structure and function of neurons. However, there is insufficient research on this aspect.