Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human DIAPH1 293 Cell Lysate

Cat.No. : DIAPH1-6925HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : DIAPH1 diaphanous homolog 1 (Drosophila) [ Homo sapiens ]
Official Symbol : DIAPH1
Synonyms : DIAPH1; diaphanous homolog 1 (Drosophila); DFNA1, diaphanous (Drosophila, homolog) 1; protein diaphanous homolog 1; hDIA1; LFHL1; diaphanous-related formin 1; diaphanous-related formin-1; DIA1; DRF1; DFNA1; FLJ25265;
Gene ID : 1729
mRNA Refseq : NM_001079812
Protein Refseq : NP_001073280
MIM : 602121
UniProt ID : O60610
Chromosome Location : 5q31
Pathway : Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; G13 Signaling Pathway, organism-specific biosystem; Regulation of actin cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, conserved biosystem; RhoA signaling pathway, organism-specific biosystem; Shigellosis, organism-specific biosystem;
Function : Rho GTPase binding; actin binding; binding; ion channel binding; profilin binding; receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (0)

Write a review

Ask a Question for All DIAPH1 Products

Required fields are marked with *

My Review for All DIAPH1 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /

Stay Updated on the Latest Bioscience Trends