Recombinant Human DHFR 293 Cell Lysate
Cat.No. : | DHFR-6946HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for dihydrofolate reductase (DHFR) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | DHFR dihydrofolate reductase [ Homo sapiens ] |
Official Symbol : | DHFR |
Synonyms : | DHFR; dihydrofolate reductase; DYR; DHFRP1; |
Gene ID : | 1719 |
mRNA Refseq : | NM_000791 |
Protein Refseq : | NP_000782 |
MIM : | 126060 |
UniProt ID : | P00374 |
Chromosome Location : | 5q11.2-q13.2 |
Pathway : | Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; E2F mediated regulation of DNA replication, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem; |
Products Types
◆ Recombinant Protein | ||
DHFR-2586H | Recombinant Human DHFR Protein, GST-tagged | +Inquiry |
DHFR-0617H | Recombinant Human DHFR Protein (M1-D187), Tag Free | +Inquiry |
DHFR-0618H | Recombinant Human DHFR Protein (M1-D187), His tagged | +Inquiry |
DHFR-755H | Recombinant Human DHFR Protein, His (Fc)-Avi-tagged | +Inquiry |
DHFR-2354M | Recombinant Mouse DHFR Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
DHFR-353HCL | Recombinant Human DHFR cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-2100 | Dihydrofolate Reductase Inhibitor Screening Kit | +Inquiry |
Kit-2097 | Dihydrofolate Reductase Colorimetric Activity Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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