Recombinant Human C5 293 Cell Lysate
Cat.No. : | C5-8020HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for complement component 5 (C5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | C5 complement component 5 [ Homo sapiens ] |
Official Symbol : | C5 |
Synonyms : | C5; complement component 5; complement C5; CPAMD4; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; FLJ17816; FLJ17822; MGC142298; |
Gene ID : | 727 |
mRNA Refseq : | NM_001735 |
Protein Refseq : | NP_001726 |
MIM : | 120900 |
UniProt ID : | P01031 |
Chromosome Location : | 9q33-q34 |
Pathway : | Activation of C3 and C5, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Complement Activation, Classical Pathway, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; |
Function : | C5a anaphylatoxin chemotactic receptor binding; chemokine activity; endopeptidase inhibitor activity; receptor binding; |
Products Types
◆ Recombinant Protein | ||
C5-344H | Recombinant Human C5 Protein, His-tagged | +Inquiry |
C5-0077H | Recombinant Human C5 Protein, GST-Tagged | +Inquiry |
C5-348P | Recombinant Pig C5 Protein, His-tagged | +Inquiry |
C5-0053H | Recombinant Human C5 Protein (G981-Q1306), His tagged | +Inquiry |
C5-575H | Recombinant Human C5 Protein (Gln19-Cys1676), His-FLAG-tagged | +Inquiry |
◆ Native Protein | ||
C5-10540H | Active Native Human C5 | +Inquiry |
C5-53H | Native Human Complement C5 | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, C5 inhibition has shown promise in treating multiple sclerosis by modulating the inflammatory response and reducing damage to the central nervous system.
C5 can be targeted using monoclonal antibodies, such as eculizumab, which inhibits its activity. This approach has been successful in managing various complement-related disorders.
In PNH, a mutation leads to uncontrolled complement activation, resulting in the destruction of red blood cells. Inhibiting C5 can prevent this process and improve clinical outcomes.
C5 inhibition can increase susceptibility to certain infections. Close monitoring and appropriate preventive measures are essential to manage the infection risk in patients receiving C5-targeted therapies.
In rheumatoid arthritis, C5 contributes to inflammation and joint damage. Targeting C5 in treatment may help mitigate the inflammatory response.
Customer Reviews (3)
Write a reviewWhether I am studying protein interactions, cellular localization, or signaling pathways, the C5 protein consistently delivers reliable and reproducible data, enabling significant scientific advancements.
Its superior purity and stability ensure reliable and accurate results, instilling confidence in my research.
Their assistance has been invaluable in streamlining my experiments and overcoming obstacles effectively.
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