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Recombinant Human DHFR cell lysate

Cat.No.: DHFR-353HCL
Species: Hamster
Tissue Type: Ovary
Preparation method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
Size: 200 ug
Recommended Usage: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer: In modified RIPA Lysis Buffer.
Applications: Western Blot;Immunoprecipitation;
Gene Name: DHFR dihydrofolate reductase [ Homo sapiens ]
Official Symbol: DHFR
Synonyms: DYR; DHFRP1
Gene ID: 1719
mRNA Refseq: NM_000791
Protein Refseq: NP_000782
MIM: 126060
UniProt ID: P00374
Chromosome Location: 5q11.2-q13.2
Pathway: Cell Cycle, organism-specific biosystem; Defective AMN causes hereditary megaloblastic anemia 1, organism-specific biosystem; Defective GIF causes intrinsic factor deficiency, organism-specific biosystem

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