Recombinant Rat Egf

Cat.No.: Egf-636R
Product Overview: Epidermal Growth Factor Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6151 Dalton. The Rat EGF is purified by proprietary chromatographic techniques.
Description: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
Source: E.coli
Species: Rat
Form: Sterile Filtered White lyophilized (freeze-dried) powder. Rat EGF was lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in PBS, pH 7.4.
Bio-activity: The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of >10,000,000 units/mg.
AA Sequence: NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR.
Purity: Greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
Usage: The products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Stability: Lyophilized Rat EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to a
Reconstitution: It is recommended to reconstitute the lyophilized Rat EGF in sterile water not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Gene Name: Egf?epidermal growth factor [?Rattus norvegicus?(Norway rat) ]
Official Symbol: Egf
Synonyms: Egf; epidermal growth factor; pro-epidermal growth factor
Gene ID: 25313
mRNA Refseq: NM_012842
Protein Refseq: NP_036974
UniProt ID: P07522
Chromosome Location: 2q42-q43
Pathway: Cytokine-cytokine receptor interaction; EGFR1 Signaling Pathway; ErbB signaling pathway
Function: calcium ion binding; epidermal growth factor receptor binding; growth factor activity

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