||Recombinant Human GST-tagged Aryl hydrocarbon receptor ligand-binding domain (AhR-LBD) consists of a 218 a.a. GST tag, a 16 a.a. flexible linker and a 207 a.a. Aryl hydrocarbon receptor ligand binding domain, Acc# NP_001612.1, amino acids 231-437. Human Aryl hydrocarbon receptor ligand-binding domain in complex with human Hsp90 was produced in insect cells co-infected with two recombinant baculoviruses. GST tagged AhR-LBD and Hsp90 are the major components of the complex.
||In the cytoplasm Aryl hydrocarbon receptor is found in complex with Hsp90. Complex of Aryl hydrocarbon receptor with Hsp90 is essential for ligand-dependent activation of the receptor.
||Sf9 insect cells
||20 mM Na-P pH 8.0, 40 mM KCl, 20 mM Na2MoO4, 7 mM reduced glutathione, 15% glycerol.
||Development of Ligand binding and coactivator peptide recruitment assays.
||Immediately store at -80℃. Avoid freeze-thaw cycles.While working, please keep sample on ice.