Recombinant Full Length Human AHR Protein, GST-tagged

• Cat.No.: AHR-603HF
• Product Overview: Recombinant full length Human AHR(1 a.a. - 848 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes.
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Molecular Mass: 122.5 kDa
• Protein Length: 848 amino acids
• AA Sequence: MNSSSANITY ASRKRRKPVQ KTVKPIPAEG IKSNPSKRHR DRLNTELDRL ASLLPFPQDV INKLDKLSVL RLSVSYLRAK SFFDVALKSS PTERNGGQDN CRAANFREGL NLQEGEFLLQ ALNGFVLVVT TDALVFYASS TIQDYLGFQQ SDVIHQSVYE LIHTEDRAEF QRQLHWALNP SQCTESGQGI EEATGLPQTV VCYNPDQIPP ENSPLMERCF ICRLRCLLDN SSGFLAMNFQ GKLKYLHGQK KKGKDGSILP PQLALFAIAT PLQPPSILEI RTKNFIFRTK HKLDFTPIGC DAKGRIVLGY TEAELCTRGS GYQFIHAADM LYCAESHIRM IKTGESGMIV FRLLTKNNRW TWVQSNARLL YKNGRPDYII VTQRPLTDEE GTEHLRKRNT KLPFMFTTGE AVLYEATNPF PAIMDPLPLR TKNGTSGKDS ATTSTLSKDS LNPSSLLAAM MQQDESIYLY PASSTSSTAP FENNFFNESM NECRNWQDNT APMGNDTILK HEQIDQPQDV NSFAGGHPGL FQDSKNSDLY SIMKNLGIDF EDIRHMQNEK FFRNDFSGEV DFRDIDLTDE ILTYVQDSLS KSPFIPSDYQ QQQSLALNSS CMVQEHLHLE QQQQHHQKQV VVEPQQQLCQ KMKHMQVNGM FENWNSNQFV PFNCPQQDPQ QYNVFTDLHG ISQEFPYKSE MDSMPYTQNF ISCNQPVLPQ HSKCTELDYP MGSFEPSPYP TTSSLEDFVT CLQLPENQKH GLNPQSAIIT PQTCYAGAVS MYQCQPEPQH THVGQMQYNP VLPGQQAFLN KFQNGVLNET YPAELNNINN TQTTTHLQPL HHPSEARPFP DLTSSGFL
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: AHR aryl hydrocarbon receptor [ Homo sapiens ]
• Official Symbol: AHR
• Synonyms: AHR; aryl hydrocarbon receptor; bHLHe76; AH-receptor; ah receptor; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76
• Gene ID: 196
• mRNA Refseq: NM_001621
• Protein Refseq: NP_001612
• MIM: 600253
• UniProt ID: P35869

Reviews

09/01/2022

Thumbs up for this lab protein – it's top-notch!

02/13/2022

I'm loving this lab protein – it's a research game-changer.

07/14/2021

Five stars for this incredible lab protein – highly recommend.

08/07/2018

The lab protein's performance has exceeded our expectations, and its versatility has enabled diverse applications within our research scope.

06/13/2018

We've seen remarkable results with this lab protein.

Q&As

What are the genetic and epigenetic factors that regulate AHR expression and function?

The expression and function of AHR can be influenced by genetic polymorphisms, DNA methylation patterns, and histone modifications, which may have implications in individual susceptibility to environmental toxins.

How is AHR involved in skin biology and the response to environmental toxins?

AHR is expressed in skin cells and plays a role in the response to environmental toxins, UV radiation, and the regulation of skin barrier function.

What is the role of AHR in embryonic development and organogenesis?

AHR is essential for normal embryonic development and organogenesis. It participates in the patterning and differentiation of various organs, including the heart and vasculature.

Can AHR activation affect hormonal signaling pathways?

Yes, AHR activation can interfere with hormonal signaling pathways, leading to cross-talk with estrogen receptor signaling, and affecting hormonal homeostasis.

How does AHR influence cellular proliferation and apoptosis?

AHR can impact cell cycle progression and apoptosis by modulating the expression of genes involved in these processes, which can have implications in cancer biology.

What role does AHR play in the immune system and inflammation?

AHR is involved in the regulation of immune responses and inflammation by affecting the differentiation and function of immune cells, such as T cells and dendritic cells.

How does AHR contribute to xenobiotic metabolism and detoxification?

AHR activation induces the expression of cytochrome P450 enzymes, particularly CYP1A1 and CYP1B1, which metabolize and detoxify various environmental pollutants and xenobiotics.

What are the downstream targets of AHR signaling, and how is gene expression regulated by AHR?

AHR forms a complex with co-activators or co-repressors upon ligand binding, leading to the modulation of gene expression. Downstream targets include cytochrome P450 enzymes (CYP1A1, CYP1B1), phase II detoxification enzymes, and cytokines.

How does AHR activation occur, and what are its endogenous ligands?

AHR activation occurs upon binding to specific ligands, including polycyclic aromatic hydrocarbons (PAHs), halogenated aromatic hydrocarbons (HAHs), and tryptophan derivatives, such as indole-3-carbinol. Endogenous ligands are derived from cellular metabolism and the diet.

How is AHR activity regulated post-translationally, and what are the protein-protein interactions that modulate its function?

AHR activity can be regulated through various post-translational modifications, including phosphorylation and ubiquitination. Protein-protein interactions with chaperones and co-regulators also influence AHR function.