Active Recombinant Human PGF, mFc-tagged

• Cat.No.: PGF-28H
• Product Overview: Active Recombinant Human PGF (Met1-Arg170) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: mFc
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Immobilized human PGF-mFc at 10 μg/mL (100 μL/well) can bind human VEGFR1-His. The EC50 of human VEGFR1-His is 0.19-0.43 μg/mL.
  2. Labeled biotin to PLGF / PGF Protein, Human, Recombinant (Fc Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PLGF / PGF Protein, Human, Recombinant (Fc Tag), Biotinylated bound to Conbercept was 4.5 nM.
• Molecular Mass: The recombinant human PGF consists 386 amino acids and predicts a molecular mass of 43.7 kDa.
• Protein Length: Met1-Arg170
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PGF placental growth factor [ Homo sapiens ]
• Official Symbol: PGF
• Synonyms: PGF; placental growth factor; PGFL, placental growth factor, vascular endothelial growth factor related protein , placental growth factor like; placenta growth factor; D12S1900; PlGF; PLGF; PlGF 2; SHGC 10760; placental growth factor-like; placental growth factor, vascular endothelial growth factor-related protein; PGFL; PlGF-2; SHGC-10760
• Gene ID: 5228
• mRNA Refseq: NM_001207012
• Protein Refseq: NP_001193941
• MIM: 601121
• UniProt ID: P49763