Recombinant Human Hypoxia-Inducible Factor 1, Alpha Subunit Inhibitor

• Cat.No.: HIF1AN-361H
• Product Overview: Recombinant human HIF1AN protein was expressed in E.coli and purified by using conventional chromatography techniques, 40.2 kDa (349 aa).

Specification

• Cat. No.: HIF1AN-361H
• Description: Hypoxia-inducible factor 1, alpha subunit inhibitor (HIF1AN), a member of the Fe2+ and 2-oxoglutarate-dependent dioxygenase superfamily, is protein that hydoxylates a specific asparagine residue (Asn-803) within the HIF1α C-terminal transactivation domain. In normoxia, the HIF1AN-mediated hydroxylation of the HIF1α transactivation domain results in blockage of the HIF1α-p300/CBP interaction and represses transcriptional activity of HIF1α-targeted genes.
• Source: Escherichia Coli.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0).
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSAS THKF LYYDEKKMAN FQNFKPRSNR EEMKFHEFVE KLQDIQQRGG EERLYLQQTL NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY DEQQNFFAQI KGYKRCILFP PDQFECLYPY PVHHPCDRQS QVDFDNPDYE RFPNFQNVVG YETVVGPGDV LYIPMYWWHH IESL LNGGIT ITVNFWYKGA PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: HIF1AN hypoxia inducible factor 1, alpha subunit inhibitor [ Homo sapiens ]
• Synonyms: HIF1AN; DKFZp762F1811; FIH1; FLJ20615; FLJ22027; hypoxia inducible factor 1, alpha subunit inhibitor; OTTHUMP00000020284; factor inhibiting HIF1; peptide-aspartate beta-dioxygenase; DKFZp762F1811; EC 1.14.11.16; FIH-1; Factor inhibiting HIF-1; Hypoxia-inducible factor asparagine hydroxylase; factor inhibiting HIF1; hypoxia-inducible factor 1, alpha subunit inhibitor.
• Gene ID: 55662
• mRNA Refseq: NM_017902
• Protein Refseq: NP_060372
• MIM: 606615
• UniProt ID: Q9NWT6
• Chromosome Location: 10q24
• Function: iron ion binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two; atoms of oxygen; peptide-aspartate beta-dioxygenase activity; protein binding.