Native Human MMP-9/TIMP-1 Complex

• Cat.No.: MMP9-41H
• Product Overview: Matrix Metalloproteinase 9 – TIMP-1 complex (MMP-9-TIMP-1, Gelatinase B, 92 kDa Type IV Collagenase, EC 3.4.24.35). Prepared from human blood certified to be negative for HBsAg and for antibodies to HIV and HCV.

Specification

• Source: Human neutrophil granulocytes (Buffy Coat)
• Species: Human
• Form: Liquid, in 50 mM Tris/HCl, pH 7.0, 200 mM NaCl, 5 mM CaCl2, 1 μM ZnCl2, 0.05% NaN3, 0.05% Brij 35.
• Purity: >95%, homogenous by electrophoresis and Western Blotting analysis
• Unit Definition: The specific activity can be assayed with the synthetic substrate N-(2,4)-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg (Dnp-petide) (Masui et al. 1977). For maximal activation the progelatinase B should be incubated with 100 μg/ml TPCK-trypsin for 30 min. at 37°C, and then the Dnp-peptide should be added. If required the trypsin can be inhibited with 1 μl of aprotinin solution (1 mg/ml) and incubated for 10 min at 25°C. One unit MMP catalyzes the hydrolysis of 1 μmol Dnp peptide/min at 37°C and pH 7.0.
• Storage: The enzyme can be kept at -80°C for many month, at -20°C for several weeks and at - 4°C for at least 1 week without significant loss of activity. Repeated freezing and thawing must be avoided.

Gene Information

• Gene Name: MMP9 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) [ Homo sapiens ]
• Official Symbol: MMP9
• Synonyms: MMP9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); CLG4B, matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase) , matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); GELB; CLG4B; MMP-9; MANDP2
• Gene ID: 4318
• mRNA Refseq: NM_004994
• Protein Refseq: NP_004985
• MIM: 120361
• UniProt ID: P14780
• Chromosome Location: 20q12-q13
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem
• Function: collagen binding; fibronectin binding; metal ion binding; metalloendopeptidase activity; peptidase activity; protein binding; protein complex binding; zinc ion binding