Active Recombinant Human GZMM protein, His-tagged
Cat.No. : | GZMM-98H |
Product Overview : | Recombinant Human GZMM protein(Ile26~Ala257), fused with N-terminal His tag, was expressed in E. coli. |
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Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose |
Bio-activity : | GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets α-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2×107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37°C for 4h. Samples were immunoblotted using Abs β-actin as control, and Ezrin to detect the enzyme activity. The results were shown below. It is obvious that recombinant human GZMM cleaved Ezrin. |
Molecular Mass : | 26.3kDa |
Protein Length : | Ile26~Ala257 |
AA Sequence : | MGHHHHHHSGSEF-IIGGR EVIPHSRPYM ASLQRNGSHL CGGVLVHPKW VLTAAHCLAQ RMAQLRLVLG LHTLDSPGLT FHIKAAIQHP RYKPVPALEN DLALLQLDGK VKPSRTIRPL ALPSKRQVVA AGTRCSMAGW GLTHQGGRLS RVLRELDLQV LDTRMCNNSR FWNGSLSPSM VCLAADSKDQ APCKGDSGGP LVCGKGRVLA RVLSFSSRVC TDIFKPPVAT AVAPYVSWIR KVTGRSA |
Purity : | >92% |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vorte. |
Gene Name : | GZMM granzyme M (lymphocyte met-ase 1) [ Homo sapiens ] |
Official Symbol : | GZMM |
Synonyms : | GZMM; granzyme M (lymphocyte met-ase 1); granzyme M; LMET1; lymphocyte met ase 1; MET1; met-ase; HU-Met-1; lymphocyte met-ase 1; Met-1 serine protease; natural killer cell granular protease; |
Gene ID : | 3004 |
mRNA Refseq : | NM_005317 |
Protein Refseq : | NP_005308 |
MIM : | 600311 |
UniProt ID : | P51124 |
Products Types
◆ Recombinant Protein | ||
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Gzmm-652R | Recombinant Rat Gzmm Protein, His/GST-tagged | +Inquiry |
Gzmm-651M | Recombinant Mouse Gzmm Protein, His/GST-tagged | +Inquiry |
GZMM-3350H | Recombinant Human GZMM Protein (Ile26-Ala257), His tagged | +Inquiry |
◆ Lysates | ||
GZMM-5666HCL | Recombinant Human GZMM 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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