Active Recombinant Rat Il17ra, His-tagged

• Cat.No.: Il17ra-7453R
• Product Overview: Recombinant Rat Il17ra (NP_001101353.2) (Met1-Pro320), fused with a polyhistidine tag at the C-terminus, was expressed in human cells.

Specification

• Source: Human Cells
• Species: Rat
• Tag: His
• Predicted N Terminal: Ser 32
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized rat IL17RA-His at 10 μg/ml (100 μl/well) can bind biotinylated human IL17A, The EC50 of biotinylated human IL17A is 5-11.8 ng/ml.2.Measured by its binding ability in a functional ELISA. Immobilized mouse IL17a at 10 μg/ml (100 μl/well) can bind rat IL17RA-His, The EC50 of rat IL17RA-His is 0.44-1.02 μg/mL.
• Molecular Mass: The recombinant rat IL17RA comprises 300 amino acids and predicts a molecular mass of 34.1 kDa. The apparent molecular mass of the recombinant protein is approximately 52-58 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC
• Storage: Store it under sterile conditions at -20ºC to -80ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Il17ra interleukin 17 receptor A [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Il17ra
• Synonyms: Il17ra; interleukin 17 receptor A; Il17r; interleukin-17 receptor A
• Gene ID: 312679
• mRNA Refseq: NM_001107883
• Protein Refseq: NP_001101353
• Chromosome Location: 4q42
• Pathway: Cytokine-cytokine receptor interaction
• Function: interleukin-17 receptor activity; molecular_function

Reviews

01/23/2021

Effective in T-cell activation.

07/12/2019

Suitable for co-immunoprecipitation.

03/17/2017

High solubility.

Q&As

What experimental techniques are employed to investigate the interaction between IL17RA and various IL17 family members, and how is their binding specificity determined?

Surface plasmon resonance, co-immunoprecipitation, and mutagenesis studies reveal IL17RA's binding partners and affinity.

Can you discuss the experimental designs used to examine the crosstalk between IL17RA signaling and other immune pathways, such as TNF-α or IFN-纬 pathways?

Cross-pathway signaling assays, gene expression profiling, and functional rescue experiments unveil IL17RA's interaction networks.

What strategies are utilized to explore the contribution of IL17RA to tissue remodeling processes, such as angiogenesis and fibrosis?

Angiogenesis assays, fibroblast activation assays, and tissue remodeling models illuminate IL17RA's role in tissue repair.

How is the potential of IL17RA as a therapeutic target evaluated in experimental settings, and what challenges exist in translating these findings to clinical applications?

Animal models, pharmacological interventions, and toxicity assessments evaluate IL17RA-targeted therapies and translational challenges.

How do researchers study the involvement of IL17RA in autoimmune disorders through patient samples, and what insights have been gained?

Patient cohorts, clinical data analysis, and immune profiling provide insights into IL17RA's relevance in autoimmune diseases.

How do researchers study the role of IL17RA in the regulation of immune cell recruitment and infiltration in inflammatory tissues?

Flow cytometry, in vivo imaging, and chemotaxis assays characterize immune cell responses guided by IL17RA activation.

Can you elaborate on the methods used to dissect the intracellular signaling pathways triggered by IL17RA activation, especially in the context of autoimmune diseases?

Phosphorylation assays, gene expression analysis, and knockout models dissect IL17RA-mediated signaling cascades.

Could you explain the experimental approaches employed to assess the impact of IL17RA inhibition on chronic inflammatory diseases in preclinical models?

Disease models, antibody-based treatments, and inflammatory biomarker assessments elucidate IL17RA's therapeutic potential.

What molecular methods are employed to understand the genetic and epigenetic regulation of IL17RA expression in immune cells?

ChIP-sequencing, promoter analysis, and epigenetic modification studies uncover IL17RA gene regulation mechanisms.

What techniques are used to investigate the role of IL17RA in maintaining epithelial barrier integrity and preventing microbial invasion?

Epithelial barrier assays, microbiome analysis, and infection models decipher IL17RA's role in mucosal defense.