Recombinant Mouse Gpc3, His-tagged Gpc3-5627M

Recombinant Mouse Gpc3, His-tagged

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Recombinant Mouse Gpc3, His-tagged

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Cat.No. : Gpc3-5627M
Product Overview : Recombinant Mouse Gpc3(Gln25-Met557 & Ser358-Met557), fused with a C-terminal 6-His tag, was expressed in Mouse NSO Cells.
Description : Glypican-3 is a protein that in humans is encoded by the GPC3 gene. The protein encoded by this gene is a member of the glypican family.
Source : Mouse NS0 Cells
Species : Mouse
Tag : His
Predicted N Terminal : Gln25 predicted (No result obtained, sequencing might be blocked) & Ser358
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by its binding ability in a functional ELISA. When Recombinant Mouse Glypican 3 is coated at 5 µg/mL (100 µL/well), the concentration of recombinant human FGF basic that produces 50% of the optimal binding response is found to be approximately 0.75 - 3.75 ng/mL.
Molecular Mass : Predicted Molecular Mass: 61.3 kDaSDS-PAGE: 66-98 kDa & 30-40 kDa, reducing conditions
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 ºC as supplied.1 month, 2 to 8 ºC under sterile conditions after reconstitution.3 months, -20 to -70 ºC under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 µg/mL in PBS.
Gene Name : Gpc3 glypican 3 [ Mus musculus (house mouse) ]
Official Symbol : Gpc3
Synonyms : Gpc3; glypican 3; OCI-5; glypican-3
Gene ID : 14734
mRNA Refseq : NM_016697
Protein Refseq : NP_057906
UniProt ID : Q8CFZ4
Chromosome Location : X; X A3.3
Pathway : A tetrasaccharide linker sequence is required for GAG synthesis; Defective B4GALT7 causes EDS; Defective CHST3 causes SEDCJD
Function : heparan sulfate proteoglycan binding; peptidase inhibitor activity; peptidyl-dipeptidase inhibitor activity; protein binding

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