Active Recombinant Mouse Mfge8, His-tagged

• Cat.No.: Mfge8-407M
• Product Overview: Recombinant Mouse Mfge8(Ala23-Cys463), fused with a C-terminal 6-His tag, was expressed in Mouse NS0 Cells.

Specification

• Description: Milk fat globule-EGF factor 8 protein (Mfge8), also known as lactadherin, is a protein which in humans is encoded by the MFGE8 gene.
• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ala23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. The ED50 for this effect is typically 10-50 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 49.8 kDaSDS-PAGE: 67 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 ºC as supplied.1 month, 2 to 8 ºC under sterile conditions after reconstitution.3 months, -20 to -70 ºC under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.

Gene Information

• Gene Name: Mfge8 milk fat globule-EGF factor 8 protein [ Mus musculus (house mouse) ]
• Official Symbol: Mfge8
• Synonyms: Mfge8; milk fat globule-EGF factor 8 protein; P47; MP47; Mfgm; SED1; MFG-E8; AA408458; AI325141; lactadherin; lactadherin; EGF/factor VIII; milk fat globule glycoprotein; sperm surface protein SP47
• Gene ID: 17304
• mRNA Refseq: NM_001045489
• Protein Refseq: NP_001038954
• UniProt ID: P21956
• Chromosome Location: 7 D3; 7 44.9 cM
• Pathway: Amyloids, organism-specific biosystem; Disease, organism-specific biosystem
• Function: integrin binding; phosphatidylethanolamine binding; phosphatidylserine binding