"NID1" Related Products

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Recombinant Human NID1 protein, His-tagged

Cat.No.: NID1-331H
Product Overview: Recombinant Human NID1(Leu29-Lys1114 (Gln1113Arg)) fused with His tag at N-terminal was expressed in NS0.
Source: NS0
Species: Human
Tag: His
Predicted N Terminal: His
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 4 x 10^4 cells/well are added to rhNidogen-1 coated plates (30 µg/mL with 100 µL/well), approximately 40-75% will adhere after one hour at 37 centigrade.
Molecular Mass: Predicted Molecular Mass: 120 kDa;SDS-PAGE: 130 kDa, reducing conditions;
Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
Gene Name: NID1 nidogen 1 [ Homo sapiens ]
Official Symbol: NID1
Synonyms: NID1; nidogen 1; NID, nidogen (enactin); nidogen-1; entactin; NID-1; enactin; NID;
Gene ID: 4811
mRNA Refseq: NM_002508
Protein Refseq: NP_002499
MIM: 131390
UniProt ID: P14543
Chromosome Location: 1q43
Function: calcium ion binding; collagen binding; extracellular matrix binding; laminin-1 binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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