Recombinant Human NID1 protein, His-tagged NID1-331H

Recombinant Human NID1 protein, His-tagged


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Recombinant Human NID1 protein, His-tagged

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Cat.No. : NID1-331H
Product Overview : Recombinant Human NID1(Leu29-Lys1114 (Gln1113Arg)) fused with His tag at N-terminal was expressed in NS0.
Source : NS0
Species : Human
Tag : His
Predicted N Terminal : His
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 4 x 10^4 cells/well are added to rhNidogen-1 coated plates (30 µg/mL with 100 µL/well), approximately 40-75% will adhere after one hour at 37 centigrade.
Molecular Mass : Predicted Molecular Mass: 120 kDa;SDS-PAGE: 130 kDa, reducing conditions;
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage : Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 μg/mL in sterile PBS.
Gene Name : NID1 nidogen 1 [ Homo sapiens ]
Official Symbol : NID1
Synonyms : NID1; nidogen 1; NID, nidogen (enactin); nidogen-1; entactin; NID-1; enactin; NID;
Gene ID : 4811
mRNA Refseq : NM_002508
Protein Refseq : NP_002499
MIM : 131390
UniProt ID : P14543
Chromosome Location : 1q43
Function : calcium ion binding; collagen binding; extracellular matrix binding; laminin-1 binding;

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