Recombinant Bovine SPAM1, His-tagged SPAM1-358B

Recombinant Bovine SPAM1, His-tagged

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Recombinant Bovine SPAM1, His-tagged

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Cat.No. : SPAM1-358B
Product Overview : Recombinant Bovine SPAM1 (Accession # AAI10184) Met1-Thr497, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO cells
Species : Bovine
Tag : His
Predicted N Terminal : Leu36
Form : Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity : Measured by its ability to hydrolyze S-35 labeled bovine cartilage chondroitin sulfate.<20 ng of recombinant human SPAM1 is required for the 50% hydrolysis of 10 µg chondroitin sulfate, as measured under the described conditions.
Molecular Mass : Recombinant Bovine SPAM1, His-tagged has a calculated MW of 53 kDa. In SDS-PAGE migrates as 60-70 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Bos taurus (cattle) ]
Official Symbol : SPAM1
Synonyms : SPAM1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); PH-20; sperm adhesion molecule 1; hyaluronidase, zona pellucida binding; NP_001008413.3; EC 3.2.1.35
Gene ID : 353352
mRNA Refseq : NM_001008413
Protein Refseq : NP_001008413
MIM :
Chromosome Location : 4

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