Recombinant Human EGF, His-tagged EGF-470H

Recombinant Human EGF, His-tagged

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Recombinant Human EGF, His-tagged

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Cat.No. : EGF-470H
Product Overview : Recombinant Human EGF (Accession # CAA28240) Met1-Arg1023, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Human
Tag : His
Predicted N Terminal : Ser21
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.The ED50 for this effect is typically 1-5 ng/mL.
Molecular Mass : Recombinant Human EGF, His-tagged has a calculated MW of 112 kDa. In SDS-PAGE migrates as 138-145 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : EGF epidermal growth factor [ Homo sapiens ]
Official Symbol : EGF
Synonyms : EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4;
Gene ID : 1950
mRNA Refseq : NM_001178130
Protein Refseq : NP_001171601
MIM : 131530
UniProt ID : P01133
Chromosome Location : 4q25

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