Active Recombinant Human FGF1, Met-tagged

• Cat.No.: FGF1-619H
• Product Overview: Recombinant Human FGF1 (Accession # NP_000791) Phe16-Asp155, fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120.The ED50 for this effect is typically 0.1-0.3 ng/mL in the presence of 10 µg/mL of heparin.
• Molecular Mass: Recombinant Human FGF1, Met-tagged has a calculated MW of 15.5 kDa.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: ITGA6 integrin, alpha 6 [ Homo sapiens ]
• Official Symbol: ITGA6
• Synonyms: ITGA6; integrin, alpha 6; integrin alpha-6; CD49f; integrin alpha6B; CD49 antigen-like family member F; VLA-6; ITGA6B; FLJ18737; DKFZp686J01244
• Gene ID: 3655
• mRNA Refseq: NM_000210
• Protein Refseq: NP_000201
• MIM: 147556
• UniProt ID: P23229
• Chromosome Location: 2q31.1