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Recombinant Human FCER1A, His-tagged

Cat.No.: FCER1A-725H
Product Overview: Recombinant Human FCER1A (Accession # NP_001992) Val26-Gln205, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: His
Predicted N Terminal: Val26
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Human Fc epsilon RI alpha is immobilized at 2 µg/mL (100 µL/well), the concentration of human IgE that produces 50% of the optimal binding response is found to be approximately 0.05 - 0.2 µg/mL.
Molecular Mass: Recombinant Human FCER1A, His-tagged has a calculated MW of 21.9 kDa. In SDS-PAGE migrates as 45-58 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: FCER1A Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide [ Homo sapiens ]
Official Symbol: FCER1A
Synonyms: FCER1A; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; FCE1A; high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; FcERI;
Gene ID: 2205
mRNA Refseq: NM_002001
Protein Refseq: NP_001992
MIM: 147140
UniProt ID: P12319
Chromosome Location: 1q23

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