Active Recombinant Human IL13
Cat.No. : | IL13-1251H |
Product Overview : | Recombinant human IL-13 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 113 amino acids with two pairs of disulfate bond and having a molecular mass of approximately 12.3kDa. |
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Description : | IL-13 is an immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils. IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four α helix configuration. Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat. IL13 has diverse activities on numerous cell types. On macrophages, IL13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while down-regulating IL-1 and TNFα production by fibroblasts and endothelial cells. IL-13R-α2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies. Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13R-α2. |
Source : | E. coli |
Species : | Human |
Form : | Lyophilized from a 0.2μm filtered solution in PBS containing 0.1% HSA, pH7.4. |
Bio-activity : | Measured in a cell proliferation assay using TF-1 cell line. The specific activity shall be not less than 1 x 10^6 IU/mg. |
Endotoxin : | ≤1EU/μg, determined by the LAL method. |
Purity : | ≥97%, as determined by SDS-PAGE and HPLC method. |
Usage : | FOR RESEARCH ONLY |
Storage : | Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 100μg/ml. |
Publication : |
Cytokine and reactivity profiles in SLE patients following anti-CD19 CART therapy (2023)
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Gene Name : | IL13 interleukin 13 [ Homo sapiens ] |
Official Symbol : | IL13 |
Synonyms : | P600; IL-13; interleukin-13 |
Gene ID : | 3596 |
mRNA Refseq : | NM_002188 |
Protein Refseq : | NP_002179 |
MIM : | 147683 |
UniProt ID : | P35225 |
Chromosome Location : | 5q31 |
Pathway : | Asthma, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem |
Function : | cytokine activity; interleukin-13 receptor binding; protein binding |
Products Types
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Il13-256I | Active Recombinant Mouse Il13 Protein, His-tagged | +Inquiry |
IL13-504H | Recombinant Human IL13 Protein, Fc-tagged | +Inquiry |
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◆ Lysates | ||
IL13-001HCL | Recombinant Human IL13 cell lysate | +Inquiry |
IL13-1336MCL | Recombinant Mouse IL13 cell lysate | +Inquiry |
IL13-1894CCL | Recombinant Cynomolgus IL13 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionFlow cytometry quantifies IL13 receptor expression on cells.
Transgenic models illuminate IL13's in vivo functions.
ELISA quantifies IL13 secretion in cell cultures accurately.
siRNA knockdown uncovers IL13's specific cellular effects.
Co-immunoprecipitation identifies IL13 signaling partners.
Differential techniques distinguish IL13 and IL4 signaling pathways.
Reporter gene assays gauge IL13-driven transcription.
Microarray analysis profiles IL13-induced gene expression.
Experimental methods reveal IL13's impact on tissue remodeling.
Site-directed mutagenesis identifies key residues in IL13 receptor binding.
Customer Reviews (3)
Write a reviewEffective in Th2 cell differentiation.
Good for cytokine assays.
High yield in expression.
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