Active Recombinant Mouse Il13 Protein

• Cat.No.: Il13-087M
• Product Overview: Purified recombinant protein of Mouse interleukin 13 (Il13) without tag was expressed in E.coli.

Specification

• Description: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.
• Source: E. coli
• Species: Mouse
• Bio-activity: The ED50 as determined by the dose-dependent proliferation of TF-1 cells was > 4.0 ng/ml, corresponding to a specific activity of > 2.5 x 10^5 units/mg.
• Molecular Mass: 12.3 kDa
• AA Sequence: MPVPRSVSLPLTLKELIEELSNITQ DQTPLCNGSMVWSVDLAAGGFCVAL DSLTNISNCNAIYRTQRILHGLCNR KAPTTVSSLPDTKIEVAHFITKLLS YTKQLFRHGPF
• Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.
• Publication:
  IFN-γ Limits Immunopathogenesis but Delays Fungal Clearance during Pneumocystis Pneumonia (2023)

Gene Information

• Gene Name: Il13 interleukin 13 [ Mus musculus (house mouse) ]
• Official Symbol: Il13
• Synonyms: Il13; interleukin 13; Il-13; interleukin-13; T-cell activation protein P600
• Gene ID: 16163
• mRNA Refseq: NM_008355
• Protein Refseq: NP_032381
• UniProt ID: P20109

Reviews

11/29/2021

Effective in Th2 cell differentiation.

04/22/2020

Good for cytokine assays.

01/26/2019

High yield in expression.

Q&As

How does flow cytometry help quantify IL13 receptor expression on different cell populations?

Flow cytometry quantifies IL13 receptor expression on cells.

What are the benefits of using transgenic animal models to study IL13's in vivo functions?

Transgenic models illuminate IL13's in vivo functions.

Could you explain the role of ELISA in quantifying IL13 secretion levels in cell cultures?

ELISA quantifies IL13 secretion in cell cultures accurately.

How can siRNA knockdown experiments reveal IL13's effects on specific cellular processes?

siRNA knockdown uncovers IL13's specific cellular effects.

How does co-immunoprecipitation identify interacting partners in IL13-mediated signaling complexes?

Co-immunoprecipitation identifies IL13 signaling partners.

What techniques can differentiate IL13-mediated signaling pathways from IL4 pathways?

Differential techniques distinguish IL13 and IL4 signaling pathways.

Could you elaborate on the use of reporter gene assays to assess IL13-driven transcriptional activity?

Reporter gene assays gauge IL13-driven transcription.

Can you describe the applications of microarray analysis in profiling IL13-induced gene expression changes?

Microarray analysis profiles IL13-induced gene expression.

What experimental methods determine the impact of IL13 on tissue remodeling processes?

Experimental methods reveal IL13's impact on tissue remodeling.

How does site-directed mutagenesis help identify critical residues in IL13 receptor binding?

Site-directed mutagenesis identifies key residues in IL13 receptor binding.