Active Recombinant Human CCL21

• Cat.No.: CCL21-522H
• Product Overview: Recombinant Human CCL21 (Accession # NP_002980 (6Ckine) & P78423 (Fractalkine Mucin-like Stalk)), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Ser24
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL).The ED50 for this effect is typically 0.5 - 1.5 µg/mL.Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7.The ED50 for this effect is typically 25 - 100 ng/mL.
• Molecular Mass: Recombinant Human CCL21 has a calculated MW of 38.3 kDa. In SDS-PAGE migrates as 95-105 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CCL21 chemokine (C-C motif) ligand 21 [ Homo sapiens ]
• Official Symbol: CCL21
• Synonyms: CCL21; chemokine (C-C motif) ligand 21; SCYA21, small inducible cytokine subfamily A (Cys Cys), member 21; C-C motif chemokine 21; 6Ckine; beta chemokine exodus 2; CKb9; ECL; Efficient Chemoattractant for Lymphocytes; exodus 2; secondary lymphoid tissue chemokine; SLC; TCA4; exodus-2; beta chemokine exodus-2; beta-chemokine exodus-2; small-inducible cytokine A21; secondary lymphoid-tissue chemokine; small inducible cytokine subfamily A (Cys-Cys), member 21; SCYA21; MGC34555
• Gene ID: 6366
• mRNA Refseq: NM_002989
• Protein Refseq: NP_002980
• MIM: 602737
• UniProt ID: O00585
• Chromosome Location: 9p13