Recombinant Human FZD7 Protein, Fc-tagged


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Recombinant Human FZD7 Protein, Fc-tagged

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Cat.No. : FZD7-537H
Product Overview : Recombinant Human FZD7(Gln33 - Leu185) fused with a human IgG1 Fc tag at the C-terminus was expressed in CHO.
Description : Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway. The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance. Like other Frizzled family members, Frizzled-7 contains a divergent N-terminal signal peptide (amino acid (aa) 1 - 32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44 - 169), a variable-length linker region (aa 170 ‑ 256), a seven-pass transmembrane region (aa 257 - 549), and a variable-length C-terminal cytoplasmic domain (aa 550 - 574). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues. Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling. Within aa 33 - 185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta -Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways. During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways. In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a. It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer. Frizzled-7 mRNA has also been detected in adult heart and placenta.
Source : CHO
Species : Human
Tag : Fc
Predicted N Terminal : Gln33
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-7/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-7/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80 ng/mL.
Optimal concentrations should be determined by each laboratory for each application.
Molecular Mass : Predicted Molecular Mass: 43.3 kDa (monomer);
SDS-PAGE: 55-66 kDa, reducing conditions.
Protein length : Gln33 - Leu185
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 200 μg/mL in PBS.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Gene Name : FZD7 frizzled family receptor 7 [ Homo sapiens ]
Official Symbol : FZD7
Synonyms : FZD7; frizzled family receptor 7; frizzled (Drosophila) homolog 7 , frizzled 7, seven transmembrane spanning receptor , frizzled homolog 7 (Drosophila); frizzled-7; FzE3; fz-7; hFz7; frizzled homolog 7; Frizzled, drosophila, homolog of, 7; frizzled 7, seven transmembrane spanning receptor;
Gene ID : 8324
mRNA Refseq : NM_003507
Protein Refseq : NP_003498
MIM : 603410
UniProt ID : O75084
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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