Active Recombinant Mouse Bmp7

• Cat.No.: Bmp7-589M
• Product Overview: Recombinant Mouse Bmp7 (Accession # NP_031583) Ser292-His430, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Predicted N Terminal: Ser292
• Form: Lyophilized from a 0.2 µm filtered solution in HCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.The ED50 for this effect is typically 0.125-0.5 µg/mL.
• Molecular Mass: Recombinant Mouse Bmp7 has a calculated MW of 15.6 kDa (monomer). In SDS-PAGE migrates as 16-20 kDa under reducing conditions.
• Protein length: Ser292-His430
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Publication:
  Gremlin Promotes Peritoneal Membrane Injury in an Experimental Mouse Model and Is Associated with Increased Solute Transport in Peritoneal Dialysis Patients (2014)

Gene Information

• Gene Name: Bmp7 bone morphogenetic protein 7 [ Mus musculus ]
• Official Symbol: Bmp7
• Synonyms: BMP7; bone morphogenetic protein 7; OP-1; BMP-7; osteogenic protein 1; OP1
• Gene ID: 12162
• mRNA Refseq: NM_007557
• Protein Refseq: NP_031583