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Recombinant Mouse Fgf7

Cat.No.: Fgf7-597M
Product Overview: Recombinant Mouse Fgf7 (Accession # NP_032034) Cys32-Thr194, fused with , was produced in E. coli.
Source: E. coli
Species: Mouse
Predicted N Terminal: Cys32
Form: Lyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Bio-activity: Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802.The ED50 for this effect is typically 10 - 50 ng/mL.
Molecular Mass: Recombinant Mouse Fgf7 has a calculated MW of 18.7 kDa. In SDS-PAGE migrates as 19 kDa, reducing conditions.
Protein length: Cys32-Thr194
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: Epyc epiphycan [ Mus musculus (house mouse) ]
Official Symbol: Epyc
Synonyms: Dspg3; PG-Lb; SLRR3B; epiphycan; epiphycan; dermatan sulfate proteoglycan 3; dermatan sulphate proteoglycan 3; proteoglycan-Lb; small chondroitin/dermatan sulfate proteoglycan
Gene ID: 13516
mRNA Refseq: NM_007884.2
Protein Refseq: NP_031910.1 
MIM:
UniProt ID: P70186
Chromosome Location: 10 50.35 cM; 10 C2-C3

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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