Recombinant Mouse Nt5e Protein, His-tagged Nt5e-667M

Recombinant Mouse Nt5e Protein, His-tagged

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Recombinant Mouse Nt5e Protein, His-tagged

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Cat.No. : Nt5e-667M
Product Overview : Recombinant Mouse Nt5e (Accession # NP_035981) Trp29-Lys549, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO Cells
Species : Mouse
Tag : His
Predicted N Terminal : Trp29
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Bio-activity : Measured by its ability to hydrolyze the 5"-phosphate group from the substrate adenosine-5"-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit.The specific activity is >10,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Mouse Nt5e, His-tagged has a calculated MW of 59 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
Protein length : Trp29-Lys549
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Nt5e 5 nucleotidase, ecto [ Mus musculus ]
Official Symbol : Nt5e
Synonyms : NT5E; 5 nucleotidase, ecto; 5-nucleotidase; 5-NT; ecto-5-nucleotidase; NT; Nt5; eNT; CD73; AI447961; 2210401F01Rik;
Gene ID : 23959
mRNA Refseq : NM_011851
Protein Refseq : NP_035981
MIM :

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