Active Recombinant Mouse Nt5e Protein, His-tagged

• Cat.No.: Nt5e-667M
• Product Overview: Recombinant Mouse Nt5e (Accession # NP_035981) Trp29-Lys549, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Trp29
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Bio-activity: Measured by its ability to hydrolyze the 5"-phosphate group from the substrate adenosine-5"-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit.The specific activity is >10,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Nt5e, His-tagged has a calculated MW of 59 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
• Protein length: Trp29-Lys549
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Nt5e 5 nucleotidase, ecto [ Mus musculus ]
• Official Symbol: Nt5e
• Synonyms: NT5E; 5 nucleotidase, ecto; 5-nucleotidase; 5-NT; ecto-5-nucleotidase; NT; Nt5; eNT; CD73; AI447961; 2210401F01Rik
• Gene ID: 23959
• mRNA Refseq: NM_011851
• Protein Refseq: NP_035981