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Recombinant Zebrafish bmp4

Cat.No.: bmp4-809Z
Product Overview: Recombinant Zebrafish bmp4 (Accession # NP_571417) Ser288-Arg400, fused with an N-terminal Met, was produced in E. coli.
Source: E. coli
Species: Zebrafish
Predicted N Terminal: Met
Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Bio-activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.The ED50 for this effect is typically 0.2-1 µg/mL.
Molecular Mass: Recombinant Zebrafish bmp4, Met-tagged has a calculated MW of 12.9 kDa (monomer).
Protein length: Ser288-Arg400
Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: bmp4 bone morphogenetic protein 4 [ Danio rerio (zebrafish) ]
Official Symbol: bmp4
Synonyms: bmp-4; zbmp-4; zgc:100779; bone morphogenetic protein 4; bone morphogenetic protein 4; etID309887.17
Gene ID: 30612
mRNA Refseq: NM_131342.2
Protein Refseq: NP_571417.1 
MIM:
Chromosome Location: chromosome: 17

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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