Active Recombinant Mouse Wisp1 Protein, His-tagged

• Cat.No.: Wisp1-980M
• Product Overview: Recombinant Mouse Wisp1 (Accession # O54775) Thr23-Asn367, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Thr23
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured in a cell proliferation assay using NRK-49F rat fibroblasts. Xu, L. et al. (2000) Gene and Dev. 14:585.The ED50 for this effect is typically 5 - 20 µg/mL.
• Molecular Mass: Recombinant Mouse Wisp1, His-tagged has a calculated MW of 39.6 kDa. In SDS-PAGE migrates as 65 kDa, reducing conditions.
• Protein length: Thr23-Asn367
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Wisp1 WNT1 inducible signaling pathway protein 1 [ Mus musculus ]
• Official Symbol: Wisp1
• Synonyms: WISP1; WNT1 inducible signaling pathway protein 1; WNT1-inducible-signaling pathway protein 1; ELM-1; WISP-1; CCN family member 4; CCN4; Elm1; AW146261
• Gene ID: 22402
• mRNA Refseq: NM_018865
• Protein Refseq: NP_061353