Recombinant Mouse Tnfsf12 Protein, Met & His-tagged


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Recombinant Mouse Tnfsf12 Protein, Met & His-tagged

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Cat.No. : Tnfsf12-983M
Product Overview : Recombinant Mouse Tnfsf12 (Accession # O54907.2) Arg105-His249, fused with an N-terminal Met and a 6-His tag, was produced in E. coli.
Source : E. coli
Species : Mouse
Tag : & His
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 0.03 - 0.3 µg/mL.Measured in a cytotoxicity assay using HT-29 human colon adenocarcinoma cells Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548; Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.The ED50 for this effect is typically 0.1 - 0.4 µg/mL.
Molecular Mass : Recombinant Mouse Tnfsf12, Met & His-tagged has a calculated MW of 17 kDa.
Protein length : Arg105-His249
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Tnfsf12 tumor necrosis factor (ligand) superfamily, member 12 [ Mus musculus ]
Official Symbol : Tnfsf12
Synonyms : TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis; Dr3l; Apo3l; Dr3lg; Tweak;
Gene ID : 21944
mRNA Refseq : NM_011614
Protein Refseq : NP_035744
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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